RICE SCIENCE ›› 2013, Vol. 20 ›› Issue (6): 415-426.DOI: 10.1016/S1672-6308(13)60153-5
出版日期:
2013-11-28
发布日期:
2013-09-06
通讯作者:
S M BALACHANDRAN
基金资助:
The authors gratefully acknowledge the financial support from the Indian Council of Agriculture Research (ICAR) through a special network project for Transgenics in Crops (NPTC) (Grant No. NPTC/2006/ 0031:3038) and the Department of Biotechnology (DBT), Government of India, through Grant No. F.No.BT/AB/FG-II(Ph-II)/2009.
摘要:
A rapid and reproducible method to develop transgenic plants with enhanced transformation efficiency using Agrobacterium has been developed for the elite indica rice variety BPT 5204. Different rice calli aged from 3 to 30 d were co-cultivated with pre-incubated Agrobacterium suspension culture (LBA4404: pSB1, pCAMBIA1301) and incubated in dark for 3 d. Based on the transient GUS gene expression analysis, 6-day-old young calli showed high transformation frequency followed by 21-day-old ones. Thus, both 6- and 21-day-old calli were used for assessing the stable transformation efficiency. It was observed that the 6-day-old young transformed calli showed about 2-fold higher regeneration frequency when compared with 21-day-old calli. The transformation efficiency was enhanced for young calli to 5.9% compared with 0.8% of the 21-day-old calli. Molecular and genetic analysis of transgenic plants (T0) revealed the presence of 1–2 copies of T-DNA integration in transformants and it follows Mendalian ratio in T1 transgenic plants. From the present study, it was concluded that the development of transgenic rice plants in less duration with high regeneration and transformation efficiency was achieved in BPT 5204 by using 6-day-old young calli as explants.
P. MANIMARAN, G. RAVI KUMAR, M. RAGHURAMI REDDY, S. JAIN, T. BHASKAR RAO, S. K. MANGRAUTHIA, R. M. SUNDARAM, S. RAVICHANDRAN, S. M. BALACHANDRAN. Infection of Early and Young Callus Tissues of Indica Rice BPT 5204 Enhances Regeneration and Transformation Efficiency[J]. RICE SCIENCE, 2013, 20(6): 415-426.
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